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KMID : 1146920200500050505
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Journal of Pharmaceutical Investigation
2020 Volume.50 No. 5 p.505 ~ p.512
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In vitro and in vivo evaluation of a novel nitric oxide-releasing ointment for the treatment of methicillin-resistant Staphylococcus aureus-infected wounds
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Lee Ju-Ho
Hlaing Shwe Phyu
Cao Jiafu
Hasan Nurhasni
Yoo Jin-Wook
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Abstract
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Purpose: Nitric oxide (NO) has emerged as a novel agent for the treatment of infected wounds owing to its potent wound-healing effects and antibacterial activity against drug-resistant bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). In this study, we developed a NO-releasing ointment composed of S-nitrosoglutathione (GSNO) and polyethylene glycol (PEG) for the treatment of MRSA-infected cutaneous wounds.
Methods: The GSNO-incorporated PEG ointment (GPO) was successfully prepared by homogeneous dispersion of micronized GSNO in a PEG ointment base. High encapsulation efficiency was achieved (97.25%) via water-free fabrication processing of the GPO, resulting in minimal GSNO hydrolysis.
Results: When applied to a wound, the wound fluid triggered the degradation of GSNO and NO was released from the GPO for 24 h without an initial burst release. The GPO exhibited potent antibacterial effects against MRSA without cytotoxic effects against L929 cells. An in vivo wound healing experiment using a mouse MRSA-challenged full-thickness wound model revealed that the GPO could facilitate healing of infected wounds.
Conclusion: Thus, the GPO could be a promising NO-releasing formulation for the treatment of infected cutaneous wounds.
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KEYWORD
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Nitric oxide, S-nitrosoglutathione, Methicillin-resistant staphylococcus aureus, Infected wound healing, Ointment
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